FastQCFastQC Report
Sun 30 Aug 2020
HCWTMBGXF_n02_bulk_34.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCWTMBGXF_n02_bulk_34.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24138046
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGAAT136500.09.8774531
GGATAGT148100.09.1037971
GTGTAGA568450.08.903431145
CAAAAAT694550.08.756351
TGTAGGG1098150.08.530905145
TCGTGTT285500.08.381121145
TAGGGAA1052400.08.267889145
AGAGTGT764500.08.223105145
TAGATCT478900.08.160903145
AGTGTAG566450.08.154026145
GTGTAGG1190500.08.100587145
GAGTGTA664950.08.058416145
GTAGGGA1050300.07.953043145
GTTAAAA405750.07.8427911
GTAGATA228600.07.8028474145
AAGAGTG831850.07.7229447145
AATCTAC216200.07.6436635
GGAAAAT554500.07.542911
GGGAAAT266450.07.48137861
CGTGTAG1299150.07.451028145
Produced by FastQC (version 0.11.5)