FastQCFastQC Report
Sun 30 Aug 2020
HCWTMBGXF_n02_bulk_33.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCWTMBGXF_n02_bulk_33.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21870750
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATAGT146900.011.8435171
TCGTGTT265800.010.42056145
GTAGGGT48500.09.567993145
GGGGAAT100100.09.4869911
GTAGGGG41900.09.344636145
GTTAAAA389550.09.34182551
GTGTAGG1130200.08.64803145
TGTAGGG1039000.08.590632145
GTGTAGA531650.08.578423145
CAAAAAT680400.08.4488991
AGAGTGT714200.08.39592145
TCTCGGG116200.08.2990465145
AGTGTAG540850.08.231408145
GATAGTT195600.08.1526422
TAGATCT443300.08.145434145
GAGTGTT168100.08.109102145
ATCTCGG396350.07.9395065145
CGGTGGG108550.07.881971145
GGGAAAT252100.07.8502061
TTAAAAG448350.07.67928932
Produced by FastQC (version 0.11.5)