FastQCFastQC Report
Sun 30 Aug 2020
HCWTMBGXF_n02_bulk_32.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCWTMBGXF_n02_bulk_32.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24157721
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTAAAAGAAAAGAAACTTTAATAAATATTTTTTCCATAGATGAATGATT247560.1024765539762629No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTAAAA442950.011.799421
GGATAGT173600.011.6919911
TCGTGTT283850.011.316048145
GGTCATA121400.010.2096274
CAAAAAT768050.09.4665461
TTAAAAG509350.09.4491212
GTAGATA223100.09.294936145
GTAGGGT55400.09.292414145
TCTCGGG127800.09.247764145
GTGTAGA706750.09.171738145
AGAGTGT932150.08.953018145
CGGTGGG109450.08.877056145
GATAGTT222550.08.8589252
ATCTCGG503700.08.536162145
TAATCCC144900.08.5039229
TAGTTAA236950.08.4121784
TCGGTGG442800.08.383826145
GAGTGTT177350.08.38113145
TGGTCGC322850.08.309615145
TAGATCT578750.08.281144145
Produced by FastQC (version 0.11.5)