FastQCFastQC Report
Sun 30 Aug 2020
HCWTMBGXF_n02_bulk_31.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCWTMBGXF_n02_bulk_31.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22350235
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTAAAAGAAAAGAAACTTTAATAAATATTTTTTCCATAGATGAATGATT250710.11217331719330914No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA120000.012.6840634
GGATAGT162250.012.3306371
TCGTGTT257800.011.897124145
GTAGGGT47250.011.662625145
CGGTGGG99800.011.551839145
GTTAAAA418050.011.3052591
TAATCCC134400.011.0016569
GTCATAA151800.09.9792025
TCTCGGG114400.09.950805145
AGGTCAT141400.09.7905753
GGGGAAT98350.09.4340191
GTAGATA205600.09.416137145
GATAGTT212800.09.4007952
GAGGTCA140300.09.2991232
TTAAAAG475850.09.2305962
ATCTCGG488400.09.13028145
GAGTGTT164850.09.104707145
CAAAAAT711000.09.0634451
TAGTTAA227350.09.0221864
GTGTAGA695450.08.809989145
Produced by FastQC (version 0.11.5)