FastQCFastQC Report
Sun 30 Aug 2020
HCWTMBGXF_n02_bulk_30.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCWTMBGXF_n02_bulk_30.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23908296
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGTT296000.013.816069145
GTAGGGT58400.012.540234145
GTAGGGG47450.011.308177145
CGGTGGG115200.011.077895145
GTTAAAA409700.010.6859851
GGATAGT163600.010.6333841
GAGTGTT186900.09.854203145
GGGGAAT112500.09.4068431
GTGTAGA602500.09.230717145
TAATCCC112550.09.1446369
TCTCGGG136600.08.9708395145
GGTCATA118400.08.876244
TTAAAAG477950.08.7652882
TGTAGGG1182600.08.62074145
CCTAGTT52350.08.4456867
CAAAAAT696200.08.4332421
AGTGTAG619250.08.40017140-144
GTGTAGG1283650.08.3544655145
CGGGAAT167150.08.2826741
TGTAGAT680100.08.124175145
Produced by FastQC (version 0.11.5)