FastQCFastQC Report
Sun 30 Aug 2020
HCWTMBGXF_n02_bulk_29.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCWTMBGXF_n02_bulk_29.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23249188
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATAGT147900.010.5866111
GTAGGGT50750.010.429888145
TCGTGTT275950.010.090057145
GGTCATA114300.09.955784
GTGTAGA677400.09.697858145
GGGGAAT120850.09.537221
GTAGATA209250.09.113457145
CGGTGGG124950.09.110794145
CAAAAAT697800.09.0481241
TCTCGGG127850.08.733993145
TAATCCC127550.08.6376999
GTTAAAA406550.08.594181
AGAGTGT913050.08.433789145
TAGATCT557400.08.403464145
AGTGTAG692750.08.331522140-144
GAGTGTT177150.08.308979145
TGGTCGC307750.08.24637145
ATCTCGG499350.08.1751585145
GTGTAGG1437500.08.171466145
TGTAGAT733600.07.9764023145
Produced by FastQC (version 0.11.5)