FastQCFastQC Report
Sun 30 Aug 2020
HCWTMBGXF_n02_bulk_28.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCWTMBGXF_n02_bulk_28.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24018928
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATAGT152700.010.8235711
GTAGATA223250.09.873473145
CGGTGGG112300.09.749559145
GTGTAGA728650.09.632615145
CAAAAAT700600.09.6017991
GAGTGTT183000.09.509288145
TCGTGTT290450.09.43644145
ATCTCGG519700.09.222243145
GTAGGGT53950.09.00474145
GGTCATA116850.08.8702154
TCGGTGG448400.08.845238145
AGAGTGT978700.08.757007145
GTTAAAA406900.08.6581141
AGTGTAG742600.08.510328140-144
TGGTCGC324500.08.468614145
TTAAAGT437900.08.2264397
GTAGGGA1385950.08.203258145
GTGTAGG1567650.08.1220045145
GGGGAAT108100.08.113991
AAGAGTG1066500.08.083674145
Produced by FastQC (version 0.11.5)