FastQCFastQC Report
Sun 30 Aug 2020
HCWTMBGXF_n02_bulk_27.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCWTMBGXF_n02_bulk_27.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23544415
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTAAAAGAAAAGAAACTTTAATAAATATTTTTTCCATAGATGAATGATT247620.10517143874672615No Hit
CAAAAATTAAAGTTCAACTGTTTAATAAAAACATTGTTTTTAGAGTATAA236750.10055463259545841No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGTT282900.010.867312145
GTTAAAA439650.010.3541741
GTAGGGT51600.010.257993145
GGATAGT179900.010.1941791
TAATCCC114450.010.0698499
GGTCATA117750.09.7874294
GTGTAGA551200.09.668684145
CAAAAAT761050.09.1055791
TTAAAAG498050.08.9357422
CGGTGGG107650.08.89097145
AGTGTAG556050.08.645476145
CCTAGTT48000.08.6074477
TGTAGAT627500.08.55081145
TGTAGGG1081900.08.511495145
ACCTAGT64800.08.5010916
GGGGAAT106800.08.4161461
ATTAAAG578800.08.327786
TCTCGGG127150.08.268769145
GTGTAGG1168800.08.226073145
GAGTGTT166600.08.138716145
Produced by FastQC (version 0.11.5)