FastQCFastQC Report
Sun 30 Aug 2020
HCWTMBGXF_n02_bulk_26.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCWTMBGXF_n02_bulk_26.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23848422
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTAAAAGAAAAGAAACTTTAATAAATATTTTTTCCATAGATGAATGATT304650.12774430106947957No Hit
CAAAAATTAAAGTTCAACTGTTTAATAAAAACATTGTTTTTAGAGTATAA294440.12346309537796672No Hit
GCATAATTTAATTTTGTAGTATATATATAATTGGGATTATGACCTCGATG267820.11230093127335637No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA125050.012.9252924
TAATCCC126250.012.8601469
GTTAAAA471800.011.9839141
GGATAGT205100.010.6380711
GTCATAA165100.010.2289025
GTAGGGT54150.010.176732145
TCGTGTT282850.010.0490055145
TTAAAAG555100.010.0018312
CCATTCA123300.09.9934428
AGGTCAT152400.09.41667653
CAAAAAT802350.09.413821
GAGGTCA157700.09.3301012
TCCATTC156950.09.2362987
CTCCATT146150.09.2244166
CGGCTCT135600.09.1945561
CTCTCCA142500.09.0028014
TGTAGGG893100.08.808924145
GTGTAGG974300.08.685031145
GTGTAGA461350.08.659928145
AGTGTAG462250.08.596008145
Produced by FastQC (version 0.11.5)