FastQCFastQC Report
Sun 30 Aug 2020
HCWTMBGXF_n02_bulk_25.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCWTMBGXF_n02_bulk_25.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22530879
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATAGT153050.09.8989591
GGTCATA103450.09.388644
GTTAAAA384400.09.3158011
AGAGTGT712750.08.881093145
GTGTAGA531750.08.876931145
TGTAGGG1049750.08.544234145
TAGGGAA1006350.08.502024145
GTGTAGG1140750.08.485554145
TGTAGAT588600.08.475348145
AGTGTAG532700.08.384697145
TCGTGTT258750.08.350746145
CAAAAAT641450.08.3174771
AAGAGTG791150.08.138483145
GTAGGGA1005450.08.019248145
ATCTCGG404550.07.9937773145
TAATCCC108450.07.95335969
TTAAAAG430400.07.88137442
TAGATCT442850.07.793628145
GAGTGTA622050.07.786469145
GGAAAAT489100.07.7217661
Produced by FastQC (version 0.11.5)