FastQCFastQC Report
Sun 30 Aug 2020
HCWTMBGXF_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCWTMBGXF_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30673172
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG561790.18315353886451655No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGTCA135650.016.3312742
CGAGGTC189150.011.5892161
TCTAGAC168600.010.0419387
ATTCTAG189050.09.7979675
CTAGACT184450.09.3575811
GGGGTAT28650.08.89687251
CATTCTA223800.08.79384
GTCCCAT151100.08.6192069
AGTCACC202900.08.535756145
GGGAAAT105400.08.429721
TAATCCC320200.08.2250659
CCCGGAT54150.08.2039941
CCGGGTT54300.08.1813311
GCGGGTT55200.08.1798731
CGTACAA260100.08.1229765
CGTCCCA160850.08.0967238
TAGACTT214600.07.95685679
GGTCATA346000.07.9255764
TTCTAGA228250.07.8931049
TTCGTAC232600.07.7774463
Produced by FastQC (version 0.11.5)