FastQCFastQC Report
Sun 30 Aug 2020
HCWTMBGXF_n01_bulk_8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCWTMBGXF_n01_bulk_8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22380092
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGGTCATAATCCCAATTATATATATACTACAAAATTAAATTATGCTGT453550.202657790682898No Hit
CAAATTTATAAAATTTTTTTATAGATAGAAACCGATCTGGCTTACACCGA390240.17436925639090312No Hit
CTCCTTCATTCTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTG376470.16821646667046766No Hit
CTTCATTCTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTGTAC363630.16247922483964766No Hit
CTTTCGTACAAATTTATAAAATTTTTTTATAGATAGAAACCGATCTGGCT358380.16013338997891519No Hit
CTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTGTACAGTCAAT342550.15306013934169707No Hit
GTCTTTTCGTCCCATAAATTTATTTAAGTTTTCTTACTTAAAAAATAAAT319200.14262675953253454No Hit
TTTTATTCTTAAAATAAAAAATATTTTTTATAAACAATCCTTTCGTACAA288750.12902091734028617No Hit
ATTTTATTCTTAAAATAAAAAATATTTTTTATAAACAATCCTTTCGTACA282950.1264293283512865No Hit
ATAGATATTAATTTTATTCTTAAAATAAAAAATATTTTTTATAAACAATC270380.1208127294561613No Hit
CCCAATTATATATATACTACAAAATTAAATTATGCTGTTATCCCTAAGGT234550.10480296506377186No Hit
CATAATCCCAATTATATATATACTACAAAATTAAATTATGCTGTTATCCC227450.10163050268068602No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA336050.023.0206034
GAGGTCA346650.020.8941462
AGGTCAT350800.020.688443
TAATCCC400950.019.3117969
GTCATAA448200.017.4222375
CGAGGTC431400.016.2692281
ATAATCC538750.014.6010178
GTCCCAT355750.014.3065149
CATAATC547150.014.2710147
CGTCCCA366600.013.8632878
TCGTCCC402750.013.1051317
TTAAACC182950.013.0775697
TCTAGAC429250.012.6001017
TCATAAT651000.012.2621986
TAGATAT357900.012.0127752
GAGCACC147450.011.997066145
ATTCTAG515700.011.9217338
CGTACAA512050.011.8798145
TTGAATG247750.011.822185145
TAAACCT184400.011.7557668
Produced by FastQC (version 0.11.5)