FastQCFastQC Report
Sun 30 Aug 2020
HCWTMBGXF_n01_bulk_7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCWTMBGXF_n01_bulk_7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24212304
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGGTCATAATCCCAATTATATATATACTACAAAATTAAATTATGCTGT457610.18899894863371947No Hit
CAAATTTATAAAATTTTTTTATAGATAGAAACCGATCTGGCTTACACCGA387220.15992695284182784No Hit
CTTTCGTACAAATTTATAAAATTTTTTTATAGATAGAAACCGATCTGGCT351520.14518238330395983No Hit
CTCCTTCATTCTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTG348780.14405072726659965No Hit
CTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTGTACAGTCAAT339470.14020557481848897No Hit
CTTCATTCTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTGTAC325980.13463402739367555No Hit
GTCTTTTCGTCCCATAAATTTATTTAAGTTTTCTTACTTAAAAAATAAAT320460.13235419479286234No Hit
ATAGATATTAATTTTATTCTTAAAATAAAAAATATTTTTTATAAACAATC289950.11975316351554152No Hit
TTTTATTCTTAAAATAAAAAATATTTTTTATAAACAATCCTTTCGTACAA267850.11062557284924227No Hit
ATTTTATTCTTAAAATAAAAAATATTTTTTATAAACAATCCTTTCGTACA266500.1100680050936086No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA336950.023.1740594
GAGGTCA344300.020.8682382
AGGTCAT346400.020.783633
TAATCCC401000.019.544129
GTCATAA456350.017.3809975
GATCATG256700.015.9852705145
CGAGGTC442950.015.9269411
ATAATCC555450.014.4881168
CATAATC560800.014.4278267
CGTATGG108200.014.003882145
CGTCCCA357850.013.7158148
GTCCCAT359650.013.586729
TCTAGAC420600.012.8419717
CCAGTCC113200.012.744889145
AAGAGCC141250.012.574972145
TAGATAT378800.012.4792012
TCGTCCC416950.012.2240757
TCATAAT665100.012.1328736
GATATTA422000.012.060814
TTAAACC201450.011.69663057
Produced by FastQC (version 0.11.5)