FastQCFastQC Report
Sun 30 Aug 2020
HCWTMBGXF_n01_bulk_46.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCWTMBGXF_n01_bulk_46.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21892868
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA149650.019.1367574
GAGGTCA166900.015.725312
AGGTCAT172750.015.1088853
TAATCCC202600.014.1707189
AAGAGCC136950.012.652199145
GTCATAA245300.012.2068755
GAGCACC163700.010.540432145
CACTCCC585250.010.529497145
CGAGGTC262550.010.162711
CCCGAAT401500.010.075783145
ACTCCCG541600.09.571005145
TCTAGAC189350.09.4573777
CATAATC327550.09.2077767
TCCCGAA497200.09.200858145
CTAGACT204900.09.1649821
ATTCTAG225900.08.9541238
ATAATCC329750.08.9483868
TCACTCC670450.08.856205145
ATCGGGG156100.08.824311145
CAGTCAC767600.08.594815145
Produced by FastQC (version 0.11.5)