FastQCFastQC Report
Sun 30 Aug 2020
HCWTMBGXF_n01_bulk_45.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCWTMBGXF_n01_bulk_45.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21895259
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAATTTATAAAATTTTTTTATAGATAGAAACCGATCTGGCTCACACCGA245380.11206992344781125No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA201850.016.3429154
GAGGTCA210600.014.4588842
AGGTCAT217800.014.01425653
TAATCCC244600.013.5157879
TCTAGAC247400.012.4837157
GTCATAA302550.011.2388935
ATTCTAG299550.011.0368045
CTAGACT280200.010.8153398
AAGAGCC124750.010.809468145
CGAGGTC305450.010.0645731
CATTCTA343450.010.0271364
CACTCAT518100.09.697318145
CGTACAA387850.09.6083145
GTCCCAT242500.09.5370039
CGTCCCA249100.09.4880258
ATAATCC377300.09.4538848
TTCTAGA346900.09.3840846
TTCGTAC383000.09.16204553
TCACTCA593600.09.160082145
GAGCACC157100.09.137364145
Produced by FastQC (version 0.11.5)