FastQCFastQC Report
Sun 30 Aug 2020
HCWTMBGXF_n01_bulk_44.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCWTMBGXF_n01_bulk_44.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21679216
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGGTCATAATCCCAATTATATATATACTACAAAATTAAATTATGCTGT289860.13370409704852795No Hit
CTTCATTCTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTGTAC239570.11050676371322653No Hit
CAAATTTATAAAATTTTTTTATAGATAGAAACCGATCTGGCTTACACCGA233080.10751311302032325No Hit
CTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTGTACAGTCAAT230960.10653521787872772No Hit
CTCCTTCATTCTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTG220840.1018671523914887No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA229900.021.6021944
GAGGTCA247000.018.4628582
AGGTCAT251450.018.2802813
TAATCCC285850.017.0437569
GTCATAA334100.015.0385525
CGAGGTC331450.013.2125061
ATAATCC419000.012.1466468
CATAATC424700.011.8473027
TCTAGAC313650.011.8349657
GTCCCAT299800.011.5834569
CGTCCCA315800.011.1113418
ATTCTAG354450.010.8614755
CTAGACT351350.010.7513761
CATTCTA401050.010.5574884
AAGAGCC122500.010.061022145
CCCAATT316550.010.0322451
TCATAAT510800.09.9215526
TCGTCCC367550.09.7443497
GATATTA345050.09.6234264
GACTTCA414700.09.5806034
Produced by FastQC (version 0.11.5)