FastQCFastQC Report
Sun 30 Aug 2020
HCWTMBGXF_n01_bulk_43.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCWTMBGXF_n01_bulk_43.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22304305
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA183250.020.6921564
GAGGTCA195650.017.9353832
AGGTCAT200350.017.6956943
TAATCCC242200.015.9249029
GTCATAA281200.014.2322885
AAGAGCC136550.012.689244145
CGAGGTC307150.011.3541661
ATAATCC359500.010.9707518
CTAGACT274850.010.6308991
CTACAGC507000.010.5673485145
CATAATC369650.010.4735467
ATTCTAG286150.010.185188
TCACTAC648200.010.021403145
CCCAATT259850.09.9331721
GTCCCAT234500.09.893429
GTCACTA681000.09.836813145
CACTACA583800.09.74844145
CGTCCCA242750.09.7363188
ATCGGGG161100.09.67548145
TCTAGAC248100.09.6726267
Produced by FastQC (version 0.11.5)