FastQCFastQC Report
Sun 30 Aug 2020
HCWTMBGXF_n01_bulk_42.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCWTMBGXF_n01_bulk_42.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23816869
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA200350.016.3929144
GAGGTCA223450.013.822152
AGGTCAT225150.013.556783
TAATCCC248050.013.1815759
AGTCACC118500.011.50197145
AAGAGCC139150.011.46231145
GTCATAA298150.011.4533735
TCTAGAC221300.010.8766417
CTAGACT249850.010.592071
ATTCTAG265600.09.6632335
GAGCACC175650.09.658294145
CGAGGTC334300.09.2827091
CCAGTCA781450.09.184736145
CAGTCAC752600.09.161129145
CATTCTA307700.09.0244054
GTATGCC197750.09.018853145
GACTTCA317050.09.0098124
ATAATCC389800.08.9088918
CGTACAA405550.08.8135285
TAGACTT309400.08.6701742
Produced by FastQC (version 0.11.5)