FastQCFastQC Report
Sun 30 Aug 2020
HCWTMBGXF_n01_bulk_41.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCWTMBGXF_n01_bulk_41.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23525960
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGGTCATAATCCCAATTATATATATACTACAAAATTAAATTATGCTGT240420.1021934917852449No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA209550.021.624244
GAGGTCA221050.018.6624412
AGGTCAT233300.018.0865553
TAATCCC266400.017.0362829
GTCATAA317050.014.5896615
CGAGGTC332300.012.3715571
TCTAGAC267100.011.7802937
ATAATCC412750.011.5401678
CATAATC412600.011.3161037
ATTCTAG303050.011.029075
CTAGACT301850.010.8332051
CATTCTA354600.010.4274954
AAGAGCC137600.010.221406145
GTCCCAT271200.09.704029
CGTCCCA272250.09.5866838
GTGATCC487150.09.524551145
TCATAAT505650.09.5207526
GACTTCA374550.09.3300894
CCCAATT288150.09.234621
TAGACTT369050.09.154772
Produced by FastQC (version 0.11.5)