FastQCFastQC Report
Sun 30 Aug 2020
HCWTMBGXF_n01_bulk_40.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCWTMBGXF_n01_bulk_40.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23492431
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAATTTATAAAATTTTTTTATAGATAGAAACCGATCTGGCTCACACCGA284250.12099641795265886No Hit
CTTTCGTACAAATTTATAAAATTTTTTTATAGATAGAAACCGATCTGGCT250370.10657475167214496No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA225350.018.113134
GAGGTCA243900.015.3680792
AGGTCAT248600.014.9609423
TAATCCC278300.014.8230359
GTCATAA335100.012.548875
TCTAGAC297900.012.2415787
AAGAGCC133900.011.694999145
ATTCTAG335150.011.1841345
GTCCCAT273300.011.1416049
CTAGACT331550.010.9334968
CGTCCCA294250.010.3483458
CGAGGTC361700.010.2832421
CATAATC416850.010.2789247
ATAATCC412500.010.2290938
ACCTATA481550.010.192349145
CATTCTA384150.010.115954
TAGATAT332150.09.8442622
TCACCTA548500.09.833844145
GATATTA395000.09.6545354
CCTATAC435200.09.612003145
Produced by FastQC (version 0.11.5)