FastQCFastQC Report
Sun 30 Aug 2020
HCWTMBGXF_n01_bulk_39.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCWTMBGXF_n01_bulk_39.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23143498
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA198350.020.2864994
GAGGTCA212650.017.2857762
AGGTCAT212500.017.263863
TAATCCC254800.016.104549
GTCATAA303500.013.6164365
AGCTATG103550.012.882412145
TCTAGAC289000.012.1919067
CGAGGTC312650.011.6185741
ACCTAGC425650.011.190311145
ATAATCC384300.011.1681618
ATTCTAG328300.011.0198815
CTAGACT318500.010.8122128
AAGAGCC133000.010.574979145
CATAATC396650.010.5097797
CATTCTA379350.010.4924914
CCTAGCT394050.010.192668145
GTCCCAT270350.010.1098879
CTAGCTA380650.09.980101145
TCACCTA528400.09.755201145
CGTCCCA283200.09.7279378
Produced by FastQC (version 0.11.5)