FastQCFastQC Report
Sun 30 Aug 2020
HCWTMBGXF_n01_bulk_38.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCWTMBGXF_n01_bulk_38.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23144296
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAATTTATAAAATTTTTTTATAGATAGAAACCGATCTGGCTCACACCGA354680.15324726230601268No Hit
CTTTCGTACAAATTTATAAAATTTTTTTATAGATAGAAACCGATCTGGCT290430.1254866425835549No Hit
CGAGGTCATAATCCCAATTATATATATACTACAAAATTAAATTATGCTGT275380.11898396045401424No Hit
CTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTGTACAGTCAAT255310.11031227737495235No Hit
CTTCATTCTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTGTAC250280.10813895570640818No Hit
CTCCTTCATTCTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTG242790.10490273715821816No Hit
ATTTTATTCTTAAAATAAAAAATATTTTTCATAAACAATCCTTTCGTACA240320.10383551955954935No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA263250.019.1968614
AGGTCAT282550.016.628213
GAGGTCA282200.016.6229612
TAATCCC315400.015.9526799
GTCATAA367300.014.2325285
TCTAGAC332400.012.45437
ATAATCC455150.011.7394778
GTCCCAT304900.011.6750819
CGAGGTC396100.011.5322681
ATTCTAG370350.011.4919425
CGTCCCA316750.011.3985218
TAGATAT335900.011.2889322
CATAATC454150.011.2866327
CTAGACT371500.010.9481288
GATATTA396100.010.87295154
CATTCTA419300.010.6171854
CGTACAA468000.010.5194175
AAGAGCC134300.010.472589145
TTCGTAC466200.010.0934173
TCATAAT551900.09.9712826
Produced by FastQC (version 0.11.5)