FastQCFastQC Report
Sun 30 Aug 2020
HCWTMBGXF_n01_bulk_37.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCWTMBGXF_n01_bulk_37.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23506617
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGGTCATAATCCCAATTATATATATACTACAAAATTAAATTATGCTGT274080.11659695650803345No Hit
CTTCATTCTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTGTAC253250.10773562184639329No Hit
CTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTGTACAGTCAAT241390.10269023398815746No Hit
CTCCTTCATTCTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTG236510.100614222795224No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA228700.020.9866494
GAGGTCA246600.017.9342982
AGGTCAT249550.017.9258143
TAATCCC290950.016.5457429
GTCATAA333400.014.9834045
GTCACCC109200.012.946165145
CGAGGTC363400.012.0707871
ATAATCC425050.011.8032778
CATAATC427650.011.5451067
TCTAGAC335200.011.5282277
ATTCTAG370500.011.0564855
CTAGACT370550.010.6443031
CATTCTA420550.010.3094354
TCATAAT523500.09.8610236
TAGATAT323350.09.7760012
CCCAATT309100.09.7345141
GTCCCAT306650.09.6225019
CGTCCCA309150.09.6150418
GACTTCA441250.09.5628994
TTCTAGA434550.09.4936066
Produced by FastQC (version 0.11.5)