FastQCFastQC Report
Sun 30 Aug 2020
HCWTMBGXF_n01_bulk_36.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCWTMBGXF_n01_bulk_36.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24711453
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGGTCATAATCCCAATTATATATATACTACAAAATTAAATTATGCTGT270580.10949578723679258No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA225100.020.3883024
GAGGTCA249050.017.4087092
AGGTCAT244550.017.4029663
TAATCCC286950.015.9424749
GTCATAA335250.013.86265
TCTAGAC315800.012.1904897
ATTCTAG357750.011.3491635
ATAATCC423950.011.2352658
CGAGGTC380050.011.1607281
CTAGACT348350.010.8431268
CATAATC431450.010.7376477
AAGAGCC142050.010.615719145
CATTCTA408750.010.4651894
GTCCCAT299350.010.05081759
CGTCCCA310250.09.7210718
CCCAATT305050.09.5550381
TTCTAGA423500.09.484486
TCACTAA611750.09.480764145
GTCACTA603250.09.446099145
CTAATCG499450.09.231928145
Produced by FastQC (version 0.11.5)