FastQCFastQC Report
Sun 30 Aug 2020
HCWTMBGXF_n01_bulk_35.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCWTMBGXF_n01_bulk_35.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23574570
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA172650.017.2593254
TAATCCC209800.014.2717589
GAGGTCA185300.014.1246472
AGGTCAT194050.013.749313
GTCATAA280350.011.0686245
TCTAGAC239950.010.3937767
CTAGACT261700.010.029371
ATTCTAG281800.09.8539015
CCAGTCC116650.09.819713145
GTCACCC514400.09.766945145
AAGAGCC138150.09.76087145
ACCCAAC416150.09.668733145
CAGTCAC615200.09.557207145
CCCAACA408650.09.491366145
CACCCAA488900.09.460784145
AGTCACC569800.09.338993145
CGAGGTC284600.08.9930831
CATTCTA328250.08.9012074
ACTCCAG809000.08.809098145
ATAATCC363450.08.756918
Produced by FastQC (version 0.11.5)