FastQCFastQC Report
Sun 30 Aug 2020
HCWTMBGXF_n01_bulk_34.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCWTMBGXF_n01_bulk_34.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24138046
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA193950.014.91531854
GAGGTCA204200.012.9949022
AGGTCAT202150.012.947413
TAATCCC245400.012.0537079
AAGAGCC139550.011.84494145
TCTAGAC232100.011.0577057
CTAGACT265200.010.6351251
GTCATAA299600.010.0187245
CAGTCAC676750.09.716433145
ATTCTAG284900.09.6958635
GTCCCAT236700.09.4644479
AGTCACC622850.09.393293145
CGTACAA395950.09.1372215
CGTCCCA243450.09.0828938
CATTCTA327700.09.0267864
GTCACCA625300.08.997071145
TAGACTT335750.08.9830732
ACTCCAG876150.08.936616145
CGAGGTC313450.08.8592511
GACTTCA347850.08.8165324
Produced by FastQC (version 0.11.5)