FastQCFastQC Report
Sun 30 Aug 2020
HCWTMBGXF_n01_bulk_33.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCWTMBGXF_n01_bulk_33.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21870750
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA187650.019.5504654
GAGGTCA198200.017.1562082
AGGTCAT212400.016.3165193
TAATCCC242850.015.1656789
GTCATAA285200.013.3718765
ATGGGGG117300.011.434126145
TGGCATG241250.011.299254145
CGAGGTC298800.011.2352131
AAGAGCC133300.011.040663145
TCTAGAC264150.011.0335347
CTAGACT296000.010.7046041
CATGGCC102250.010.210064145
ATTCTAG302650.010.1813265
ATAATCC373900.09.9665288
GGGGGTT96150.09.877597145
CATAATC379150.09.8285917
ACCATGG446900.09.782193145
GAGCACC155850.09.675776145
TGGGGGG131650.09.637102145
GACTTCA360050.09.5650214
Produced by FastQC (version 0.11.5)