FastQCFastQC Report
Sun 30 Aug 2020
HCWTMBGXF_n01_bulk_32.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCWTMBGXF_n01_bulk_32.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24157721
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGGTCATAATCCCAATTATATATATACTACAAAATTAAATTATGCTGT312720.1294492969763166No Hit
CTCCTTCATTCTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTG260360.10777506702722495No Hit
CTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTGTACAGTCAAT256150.10603235296905698No Hit
CTTCATTCTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTGTAC250050.10350728034320786No Hit
CAAATTTATAAAATTTTTTTATAGATAGAAACCGATCTGGCTTACACCGA245970.10181837930821373No Hit
CTTTCGTACAAATTTATAAAATTTTTTTATAGATAGAAACCGATCTGGCT245840.10176456628503988No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA250550.022.3685474
GAGGTCA263950.019.6121672
AGGTCAT269350.019.2997683
TAATCCC310550.017.8359979
GTCATAA364400.015.49935155
CAGGCGC144150.014.887001145
CGAGGTC385500.013.3163131
ATAATCC451750.012.630238
TCTAGAC336300.012.4822697
AAGAGCC146000.012.41416145
CATAATC457700.012.3712047
ATTCTAG379600.011.5171645
CTAGACT376750.011.2776721
CCAGTCC110650.010.941962145
CATTCTA428950.010.9019884
TCATAAT560800.010.3556886
CCCAATT338900.010.2907881
GAGCACC174350.010.104492145
ACCAGGC583500.09.989537145
GACTTCA446450.09.9874574
Produced by FastQC (version 0.11.5)