FastQCFastQC Report
Sun 30 Aug 2020
HCWTMBGXF_n01_bulk_31.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCWTMBGXF_n01_bulk_31.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22350235
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGGTCATAATCCCAATTATATATATACTACAAAATTAAATTATGCTGT307280.1374840130316303No Hit
CAAATTTATAAAATTTTTTTATAGATAGAAACCGATCTGGCTTACACCGA253460.11340372931201842No Hit
CTTTCGTACAAATTTATAAAATTTTTTTATAGATAGAAACCGATCTGGCT247800.11087131746042045No Hit
CTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTGTACAGTCAAT244580.10943061672505905No Hit
CTTCATTCTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTGTAC240600.10764987482234528No Hit
CTCCTTCATTCTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTG226920.10152913381000245No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA241900.021.879484
GAGGTCA258850.018.6540952
AGGTCAT259050.018.6117043
TAATCCC286000.018.3532039
GTCATAA338600.015.7596915
CGAGGTC349150.013.7057651
GTCACCC96350.013.619353145
ATAATCC426900.012.8560958
CATAATC434400.012.6176977
CCAGTCC109400.012.259818145
GTCCCAT289050.011.186689
CTAGACT356250.011.0310241
TCTAGAC315200.010.971867
TAGATAT314000.010.7828932
GAGCACC165250.010.660914145
TCATAAT523250.010.5585566
ATTCTAG361750.010.4821415
CGTCCCA309200.010.4342178
GATATTA369400.010.3826654
AAGAGCC132650.010.274961145
Produced by FastQC (version 0.11.5)