FastQCFastQC Report
Sun 30 Aug 2020
HCWTMBGXF_n01_bulk_30.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCWTMBGXF_n01_bulk_30.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23908296
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAATTTATAAAATTTTTTTATAGATAGAAACCGATCTGGCTCACACCGA294700.12326265326479144No Hit
CTTTCGTACAAATTTATAAAATTTTTTTATAGATAGAAACCGATCTGGCT252590.10564952014982582No Hit
CGAGGTCATAATCCCAATTATATATATACTACAAAATTAAATTATGCTGT248620.10398900866879011No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA255450.017.5116844
GAGGTCA268900.015.6111072
AGGTCAT270050.015.5178773
TAATCCC309400.014.4577219
GTCATAA361550.012.8139485
AAGAGCC139000.012.048377145
TCTAGAC277550.011.5978797
CGAGGTC380300.011.0195941
TAGATAT329250.010.811892
GTCCCAT269900.010.5566159
ATAATCC443700.010.5064648
GATATTA379400.010.3765064
ATTCTAG318200.010.34441955
CGTCCCA277600.010.3160318
CTAGACT312650.010.1334668
CATAATC453600.010.0214677
GAGCACC176200.09.957283145
CATTCTA368150.09.6498364
AGTCACC683500.09.5357145
CGTACAA454550.09.3947255
Produced by FastQC (version 0.11.5)