FastQCFastQC Report
Sun 30 Aug 2020
HCWTMBGXF_n01_bulk_29.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCWTMBGXF_n01_bulk_29.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23249188
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGGTCATAATCCCAATTATATATATACTACAAAATTAAATTATGCTGT247710.10654565656228511No Hit
CTTCATTCTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTGTAC233320.10035619308510904No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA224000.019.452894
GAGGTCA237550.017.030762
AGGTCAT245650.016.380753
TAATCCC286650.015.2005629
GTCATAA319500.013.8653495
TCTAGAC296100.012.3161287
CGAGGTC351100.011.4820231
ATTCTAG333800.011.273055
CTAGACT328850.010.9791478
CATAATC420450.010.91525657
ATAATCC414850.010.8352188
CATTCTA377300.010.5497754
CAACTAA513900.09.8893585145
GTATGCC221650.09.8779745145
CCAACTA525500.09.864203145
GAGCACC174000.09.833122145
AAGAGCC145900.09.739338145
TAGATAT326900.09.5591042
TAGACTT387650.09.5569269
AGTCACC764300.09.533029145
Produced by FastQC (version 0.11.5)