FastQCFastQC Report
Sun 30 Aug 2020
HCWTMBGXF_n01_bulk_28.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCWTMBGXF_n01_bulk_28.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24018928
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA196500.019.8139554
GAGGTCA214050.016.8343752
AGGTCAT219100.016.6450483
TAATCCC259850.014.8990039
AAGAGCC143850.013.70846145
GTCATAA302400.013.3787485
TCTAGAC273200.012.0216067
CTAGACT303050.011.9868131
ATTCTAG313000.011.3968245
CGAGGTC328000.011.09711551
CATAATC397150.010.4968327
CATTCTA360000.010.4123194
ATAATCC394700.010.396528
GTATGCC232250.010.363631145
TAGACTT368550.010.2690412
CGTCCCA256500.010.2037198
GTCCCAT249050.010.1887329
CCCGGGT62300.010.1253821
GACTTCA372900.09.9743464
CAGTCAC880750.09.877754145
Produced by FastQC (version 0.11.5)