FastQCFastQC Report
Sun 30 Aug 2020
HCWTMBGXF_n01_bulk_27.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCWTMBGXF_n01_bulk_27.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23544415
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAATTTATAAAATTTTTTTATAGATAGAAACCGATCTGGCTCACACCGA360010.15290675092160924No Hit
CTTTCGTACAAATTTATAAAATTTTTTTATAGATAGAAACCGATCTGGCT326880.1388354732958963No Hit
CGAGGTCATAATCCCAATTATATATATACTACAAAATTAAATTATGCTGT310130.13172125958534114No Hit
CTTCATTCTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTGTAC280120.11897513699108683No Hit
CTCCTTCATTCTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTG278530.11829981760005505No Hit
CTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTGTACAGTCAAT266970.11338994831683012No Hit
ATTTTATTCTTAAAATAAAAAATATTTTTCATAAACAATCCTTTCGTACA259440.11019173761590594No Hit
ATAGATATTAATTTTATTCTTAAAATAAAAAATATTTTTCATAAACAATC253440.10764336255540856No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA294750.018.2026584
GAGGTCA316950.015.9897762
AGGTCAT314400.015.888893
TAATCCC352100.015.3193969
GTCATAA400250.013.7851965
TAGATAT348450.011.8393472
TCTAGAC357150.011.5911287
GAGCATG129750.011.454538145
ATAATCC486500.011.4449058
CGTACAA494050.011.4174295
GATATTA399000.011.4115234
GTCCCAT321300.011.3950279
CGAGGTC450750.011.179671
ATTCTAG397700.011.0660525
TTCGTAC483800.011.0448173
CATAATC497650.011.0429597
AAGAGCC129250.010.994021145
CGTCCCA328850.010.8688338
CTAGACT392500.010.362328
CATTCTA448900.010.15916254