FastQCFastQC Report
Sun 30 Aug 2020
HCWTMBGXF_n01_bulk_26.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCWTMBGXF_n01_bulk_26.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23848422
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGGTCATAATCCCAATTATATATATACTACAAAATTAAATTATGCTGT404060.16942840075540427No Hit
CTTTCGTACAAATTTATAAAATTTTTTTATAGATAGAAACCGATCTGGCT366840.1538214981267943No Hit
CAAATTTATAAAATTTTTTTATAGATAGAAACCGATCTGGCTCACACCGA360170.15102466737631529No Hit
CTTCATTCTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTGTAC357350.14984219920294936No Hit
CTCCTTCATTCTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTG347910.14588386602685913No Hit
ATTTTATTCTTAAAATAAAAAATATTTTTTATAAACAATCCTTTCGTACA346000.14508297446262902No Hit
TTTTATTCTTAAAATAAAAAATATTTTTTATAAACAATCCTTTCGTACAA345800.14499911147161015No Hit
CTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTGTACAGTCAAT340970.14297382023850466No Hit
ATAGATATTAATTTTATTCTTAAAATAAAAAATATTTTTTATAAACAATC336530.14111206183788597No Hit
CTGGCTCACACCGATCTAAACTCAAATCATGTAAGATTTTAATAATCGAA300760.12611316589416272No Hit
GTCTTTTCGTCCCATAAATTTATTTAAGTTTTCTTACTTAAAAAATAAAT295820.12404175001599688No Hit
ATTTTTTTATATAATAAATTAAAAATAAAATTCTATAGGGTCTTTTCGTC246440.10333597753344016No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA383400.019.2886454
GAGGTCA400100.017.0519052
AGGTCAT402400.017.0265483
TAATCCC447500.016.5251129
GTCATAA496250.014.9463195
TAGATAT387500.013.9017332
GATATTA446300.013.2561354
ATAATCC587300.012.9988448
CGAGGTC518350.012.92461
CATAATC588950.012.7532637
TCTAGAC455000.012.3330247
ATTCTAG493400.011.8873688
GTCCCAT394850.011.8247239
CGTCCCA403950.011.6301088
CATTCTA544400.011.2136664
TCATAAT683850.011.0263926
CTAGACT509850.010.8923798
TCTTAAA571300.010.736097
AGATATT534050.010.6842873
TTCTAGA557350.010.5364499
Produced by FastQC (version 0.11.5)