FastQCFastQC Report
Sun 30 Aug 2020
HCWTMBGXF_n01_bulk_25.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCWTMBGXF_n01_bulk_25.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22530879
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAATTTATAAAATTTTTTTATAGATAGAAACCGATCTGGCTCACACCGA228060.1012210841840658No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA210150.018.2503174
AGGTCAT231350.015.042293
GAGGTCA231750.015.0162942
TAATCCC266700.014.4074589
GTCATAA313100.012.5505375
TAGATAT313700.012.08727552
GATATTA377850.011.1864774
TCTAGAC257550.010.4154627
CTAGACT285650.010.3813181
ATAATCC394350.09.9644188
CGAGGTC350750.09.9221771
CATAATC404100.09.7958517
AAGAGCC125350.09.716607145
CCAGTCA656700.09.715057145
CAGTCAC636250.09.423395145
ATTCTAG297400.09.3856825
CGTACAA416500.09.2606765
CGTAGAG443950.09.259311145
CATTCTA345500.09.1911574
GTCCCAT255150.09.14944659
Produced by FastQC (version 0.11.5)