FastQCFastQC Report
Sat 5 Sep 2020
HCWTLBGXF_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCWTLBGXF_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29583707
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG934540.3158968549816965No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC137900.030.2105813
CGTCTGA139150.028.91610716
ACGTCTG141650.028.57175315
CACGTCT147200.027.73196814
CACACGT158850.026.62232412
GCACACG165400.025.94849611
ATCGGAA194300.024.4662152
CGGAAGA192450.024.3949474
TCGGAAG198400.023.7167263
GATCGGA201400.023.5825311
GAACTCC200100.019.21753521
AGAGCAC245700.018.5351098
AGCACAC242050.018.4824110
CTGAACT219550.018.08808319
TGAACTC245650.016.00970820
AACTCCA247850.015.30365222
TCTGAAC277050.014.69991118
CGGGGGG99500.014.2636771
GAGCACA347750.013.387219
GAAGAGC365450.013.1621146
Produced by FastQC (version 0.11.5)