FastQCFastQC Report
Sat 5 Sep 2020
HCWTLBGXF_n01_mb362.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCWTLBGXF_n01_mb362.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31454696
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGGT37200.013.3619321
GTCCCGT48750.012.5657181
CCCGTAT32050.012.341681
GTCCGTT47550.012.1466711
GACGTAT18350.012.01623217
CTCGTAT57000.011.9138131
GTCCGGA45300.011.2818031
CTCCGCT94100.011.2341761
GTCCGAA32550.011.0766931
CTCGCGT8000.010.9389061
CTCCGGT89550.010.7104781
GTCGCAT21950.010.525271
CTCCGAT83400.010.4509841
GTCCTAT56000.010.3763331
CTCCGTT80800.010.2674091
CCCCGAT52350.010.02994251
ACGTATA22650.09.889544518
CGTATAG21650.09.86135119
GTCCGGG57350.09.8268961
CCCGATT32950.09.4549331
Produced by FastQC (version 0.11.5)