FastQCFastQC Report
Sat 5 Sep 2020
HCWTLBGXF_n01_mb361.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCWTLBGXF_n01_mb361.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33261767
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATTA15600.013.9122733
CCCGTAT34750.013.1960781
GTCCGTT47300.012.9510591
GTCCGGT36600.012.7203471
GACGTAT23400.012.41435117
CGTATAG25450.011.68947119
GTCCCGT49050.011.1330461
ACGTATA27100.011.10690218
CGCTAGT11450.011.0058961
CTCCGGT85300.010.9159361
CTCGTAT62250.010.6842161
GTCCGAA32150.010.6702221
CTCCGCT94300.010.5422921
CCGTATT47550.010.3064372
GACTACG28250.010.28300835
TCCGCTA14650.010.2745272
GTTCGCG5201.0916483E-710.0975571
GTCGCAT23250.010.0874231
GTCCGGA48100.09.8974251
CCCGTTT72250.09.8352771
Produced by FastQC (version 0.11.5)