FastQCFastQC Report
Sat 5 Sep 2020
HCWTLBGXF_n01_mb360.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCWTLBGXF_n01_mb360.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30442359
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG17750.014.196938546
GTCCGAA29050.012.6520881
GTCCGGA44650.012.3082761
GTCCGTT44450.012.2849051
GTCCGGT33200.012.1249171
GACGTAT21100.011.94317
CCCGTAT31400.011.7051961
CTCCGCT84700.011.4889521
CGTATAG23250.011.44084919
GACTACG25000.011.19980635
CTCGTAT58600.010.6326471
GTCGCGA4005.1737225E-610.5012331
GTTCGTT36900.010.4348291
CTCCGAT83050.010.3684651
GTCGCAA15400.010.2284731
GTCCGCA49200.010.1739591
GTCCCGT44050.09.9330621
ACGTATA24750.09.89896518
CTCCGGT79900.09.7257981
GTCGTTT78200.09.7134161
Produced by FastQC (version 0.11.5)