FastQCFastQC Report
Sat 5 Sep 2020
HCWTLBGXF_n01_mb359.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCWTLBGXF_n01_mb359.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31307124
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGGT38700.013.2061231
GTCCGTT53500.013.1515131
CGTATTA15350.012.3146763
CCCGTAT33000.012.1988071
GACGTAT20000.011.54985717
CGCTAGT12000.011.3767141
CTCCGCT100650.010.7467761
GTCCGGA53050.010.4916841
CGTATAG21500.010.41849319
GTCGCAT22650.010.20021251
GTCCGAA34400.010.0741921
GACTACG23650.010.06320635
GTCCGTA30200.09.9683881
ACGTATA22950.09.91273218
GTCCCGT54500.09.8913971
GTCCGCT66800.09.799381
GTCGGAT37600.09.4961111
GTCCTAT57200.09.4244961
CCCGTCT82000.09.3916581
GTCCGCA50900.09.2843071
Produced by FastQC (version 0.11.5)