FastQCFastQC Report
Sat 5 Sep 2020
HCWTLBGXF_n01_mb358.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCWTLBGXF_n01_mb358.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41963249
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATGC1918660.45722389131499325TruSeq Adapter, Index 22 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG289850.050.94441643
CTCGTAT288050.050.7524442
TATGCCG298650.049.97065746
CGTATGC300950.049.87949844
TCTCGTA292600.049.05415741
ATGCCGT302250.048.6575347
ATCTCGT307450.047.39060640
ACGGTAG369250.045.2503832
CCGTCTT319350.044.1451450
TGCCGTC328550.043.97426248
CACGGTA383700.043.61017631
GTATGCC349100.043.35070845
CATCTCG345950.042.54152339
CGGTAGC395000.042.22963333
CGTCTTC334650.041.2797151
GCCGTCT339500.041.0611349
TCACGGT413100.040.56582330
AGTCACG418300.040.11198428
GTAGCAT405500.039.7206335
GTCACGG436450.038.4277329
Produced by FastQC (version 0.11.5)