FastQCFastQC Report
Sat 5 Sep 2020
HCWTLBGXF_n01_mb357.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCWTLBGXF_n01_mb357.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39368236
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCTCGTATGC1719630.4368064649886777TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT232800.057.65583842
TCGTATG236500.057.3161843
TCTCGTA233300.056.60215441
CGTATGC257800.056.4090844
TATGCCG262600.055.76450346
ATGCCGT258650.055.22236647
ATCTCGT247400.054.16846540
ACGAGTG337200.052.68596632
GATCTCG259400.052.60707539
CACGAGT344600.051.6967731
GTCACGA345100.051.4697429
TCACGAG350900.050.64892230
TGCCGTC274250.050.61356748
CCGTCTT265900.050.24174550
CGAGTGG360050.048.97294633
GCCGTCT271300.048.5063949
CGCGTAT29550.048.20563542
AGTCACG370850.047.98099528
GTATGCC311500.047.7857345
CGTCTTC277250.046.13990851
Produced by FastQC (version 0.11.5)