FastQCFastQC Report
Sat 5 Sep 2020
HCWTLBGXF_n01_mb356.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCWTLBGXF_n01_mb356.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34590503
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTCGTATGC886280.2562206163928868TruSeq Adapter, Index 22 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGA123750.056.14042734
GTACGAT124900.054.11033235
TACGATC119300.053.80450436
TCGTATG131550.053.55656443
CCGTACG133750.052.91121733
CTCGTAT127500.052.4029242
CGTATGC136450.051.81286244
TCTCGTA127750.051.31408741
ACCGTAC141500.050.48320432
TATGCCG141050.049.99904646
ATGCCGT143650.049.04535747
ATCTCGT137000.047.21077740
ACGATCT136400.047.0592237
CACCGTA153850.046.4990231
CGATCTC142600.045.1113438
GATCTCG143800.044.83224539
TGCCGTC159700.044.09433748
CCGTCTT163650.043.1583650
GCCGTCT169050.041.82114849
CGTCTTC175550.040.3324751
Produced by FastQC (version 0.11.5)