FastQCFastQC Report
Sat 5 Sep 2020
HCWTLBGXF_n01_mb355.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCWTLBGXF_n01_mb355.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34977574
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGATCTCGTATGC1917080.5480883265374551TruSeq Adapter, Index 21 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT284050.051.76279442
TCGTATG305650.051.7346743
CGTATGC312350.050.748244
TATGCCG311900.050.6867646
TCTCGTA278900.050.47233241
ATGCCGT317100.049.7672747
TTCGATC278150.049.65212636
ATCTCGT284750.048.50127840
CGTTTCG326550.048.34849533
TTTCGAT306550.048.2375535
GTTTCGA321550.048.02272834
TCGATCT290850.047.36372837
CCGTCTT336900.046.6865650
GATCTCG300200.046.04010439
TGCCGTC344750.045.84684848
CGATCTC302950.045.5066538
GTATGCC349950.045.3556245
ACGTTTC354100.045.0711632
GCCGTCT353500.044.57341449
CGTCTTC354100.044.5077751
Produced by FastQC (version 0.11.5)