FastQCFastQC Report
Sat 5 Sep 2020
HCWTLBGXF_n01_mb354.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCWTLBGXF_n01_mb354.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34486183
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTCGTATGC1394920.4044866316460711TruSeq Adapter, Index 20 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATGTCGTATGC369770.10722265204009386TruSeq Adapter, Index 20 (97% over 41bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG239300.057.02573443
CGTATGC259200.056.14484444
CTCGTAT194800.055.40969542
TATGCCG266450.054.99808546
ATGCCGT257750.054.88554847
TCTCGTA196300.054.30877341
ATCTCGT214150.051.48169740
TGCCGTC266100.051.42712448
CCGTCTT255650.050.53108250
GTCGTAT64100.049.96011442
GTCACGT387100.049.8634929
AGTCACG389100.049.6882928
GCCGTCT261100.048.56482749
ACGTGGC398000.048.2956332
GTATGCC307650.048.2812945
CGTCTGA412750.047.5708816
CGTCTTC256350.047.5123351
CATCTCG237150.047.37423739
ACGTCTG424300.046.42434715
ACACGTC426750.046.37099513
Produced by FastQC (version 0.11.5)