FastQCFastQC Report
Sat 5 Sep 2020
HCWTLBGXF_n01_mb353.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCWTLBGXF_n01_mb353.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38942909
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAATCTCGTATGC1573240.4039862558803709TruSeq Adapter, Index 19 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG237500.051.5633143
CTCGTAT239750.051.09399842
CGTATGC250900.050.4415344
TCTCGTA244000.050.43354841
TATGCCG255250.050.06180646
ATGCCGT249500.049.56025347
AATCTCG260450.049.10261539
ATCTCGT262050.048.3219940
TGCCGTC260100.046.1814348
CCGTCTT254600.045.43322450
GTCACGT354350.044.1013329
GCCGTCT261300.043.82625249
AGTCACG359100.043.66423428
CGTCTTC260000.042.69925751
GTATGCC303800.042.637545
CGTCTGA388450.040.76160416
CACGTGA387250.040.2731331
TCACGTG392000.039.81190530
ACGTCTG406400.039.11615415
ACACGTC410450.038.8241813
Produced by FastQC (version 0.11.5)