FastQCFastQC Report
Sat 5 Sep 2020
HCWTLBGXF_n01_mb352.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCWTLBGXF_n01_mb352.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35462319
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCTCGTATGC845630.23845874264455183TruSeq Adapter, Index 18 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG117700.054.38736343
ACGTCCG147950.054.17287432
CTCGTAT118350.054.14779742
CGTATGC125700.053.7103144
TCTCGTA118450.053.59977341
TATGCCG130900.051.49646446
ATGCCGT128150.051.4544647
TCCGCAT151450.050.81789835
CGTCCGC161050.049.59253333
GTCCGCA159750.049.44837634
ATCTCGT133950.048.59940740
CCGCATC156400.048.0011136
CGCATCT143000.047.82436837
TGCCGTC137400.047.12438648
GCCGTCT136750.044.84019549
CCGTCTT138350.044.80228450
CATCTCG148500.044.78038839
GTCACGT187700.043.1293929
TCGCGTA12500.042.2792941
CGCGTAT13050.041.8383842
Produced by FastQC (version 0.11.5)