FastQCFastQC Report
Sat 5 Sep 2020
HCWTLBGXF_n01_mb351.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCWTLBGXF_n01_mb351.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37928194
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTCGTATGC1063640.28043518233428144TruSeq Adapter, Index 3 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG154000.052.65827643
CTCGTAT150250.052.27206842
CGTATGC159200.051.07018344
TCTCGTA153400.050.5370241
TATGCCG165250.049.2639846
ATGCCGT167100.048.6557447
CGTAGAG174000.047.12857433
ACGTAGA177000.046.56707432
ATCTCGT165450.046.32747740
GATCTCG170650.044.85426739
TGCCGTC181300.044.70973248
TCACGTA187350.044.18150330
CCGTCTT185500.043.60309250
CACGTAG189100.043.47637631
GCCGTCT192050.042.1159849
CGTCTTC201250.040.13850851
GTCACGT206850.039.94877629
AGTCACG212550.039.12445428
GTATGCC215650.037.89646545
CGTCTGA235350.035.7208516
Produced by FastQC (version 0.11.5)