FastQCFastQC Report
Wed 29 Jul 2020
HCW3JBGXF_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCW3JBGXF_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17163037
Sequences flagged as poor quality0
Sequence length36
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9231395.378645982060168No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTGAC16700.020.65497816
AGTGACT16650.020.44875717
AACCGGG6800.019.62883416
GGCGAAC6550.019.46005212
TGAGGCG7100.018.9982289
GGGAACT7050.018.95234122
CCTGCCG18050.018.80276125
TGCCCTG19250.018.71726822
ATGGGCC13900.018.54816810
GAACCGG6900.018.47721715
ACCTTTG8550.018.06524513
GGGGAAC7650.017.65954621
GATAGTG19400.017.62457314
AGGCGAA7500.017.59195711
AATGGGC14850.017.359259
GATTCGG21250.017.2791927
TTGCCCT20800.017.17560421
GGGCCTA3850.017.1168561
GGTTACC4600.016.9418897
CCGAGGA21900.016.80361429
Produced by FastQC (version 0.11.5)