FastQCFastQC Report
Thu 30 Jul 2020
HCW3JBGXF_n02_28hr_males.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCW3JBGXF_n02_28hr_males.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64226769
Sequences flagged as poor quality0
Sequence length36
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3646420.5677414661790009No Hit
CGTGGGACATGAATTAACAACATTTAAAGTTTGTTA1675220.2608289387871901No Hit
ACGAGATCCGTGATTGCTTCTACAACCATTTGGACA1575340.24527779063586397No Hit
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT1361140.21192721059968Illumina RNA PCR Primer (100% over 31bp)
TCTCGCGATTCGGAGGGTTTTTGCCCTGCCGAGGAG1232770.1919402173258941No Hit
GTCGTGATTACCCGCTGAACTTAAGCATATCATTTA1207810.18805398727125758No Hit
CTGTTGAGTATGAGATAGTGACTAAAAAAAAAAAAA1049420.16339293044618203No Hit
TCTGTTACAATGAACGTTCGTTCAAATTTTTGTTTT703630.10955400854743293No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGGGA220000.024.8833121
GTGGGAC256300.024.407192
GGCCGCC54150.023.76441
GGGCCTA30500.023.6036641
TGGGACA272050.022.8290823
GATAGTG253900.022.3792314
GGCGCAT90650.022.1704751
GGGACAT284850.021.9780144
GATAGGC63700.021.7595927
AGTGACT258650.021.69017817
GTGACTA261900.021.5870818
AGGCTTG64350.021.51723930
ACGTAAG59600.021.4432812
GGCCCCG26800.021.433941
GCCGAGG249150.021.10927428
TAGTGAC271450.020.99877716
ATAGGCT66350.020.7776728
GGACATG303150.020.5726555
TAGATCT213550.020.4562130
GACATGA306050.020.4070246
Produced by FastQC (version 0.11.5)